Use of the zebrafish model as a tool to evaluate the anti-inflammatory and antioxidant activity of foods. Literature review

Abstract

The use of metabolites obtained from plants that are suitable for human consumption requires a prior evaluation to measure their effectiveness and adverse effects. Bioethics committees control the use of laboratory animals for these purposes, making working with these models imply high costs and more time to carry out laboratory tests. Hence, zebrafish have great advantages as an animal model, because in their first hours of development they are transparent, their growth is rapid, simple techniques can be used for their study, oxidative and inflammatory stress can be induced to evaluate the anti-inflammatory response and oxidative stress in a similar way to the processes that occur in humans. This work is based on identifying the analysis techniques used to evaluate the antioxidant and anti-inflammatory effect using the zebrafish model. To search for scientific articles, the Science Direct, Google Scholar and SciELO databases were used using the terms zebra fish, antioxidant, anti inflammatory, model, Danio rerio. Fifty articles were reviewed, of which thirty-three were chosen to carry out this review and were classified according to the source of plant extracts, compounds extracted from plants, chemical compounds and other sources, of each article the in vivo, in vitro analyzes were highlighted. and the treatment conditions used in zebrafish, with the aim of analyzing the most relevant techniques that can be performed in this model and knowing the complementary analyzes that can be performed on zebrafish.